Neutron Diffractometer for Biological Macromolecule Crystallography
نویسندگان
چکیده
منابع مشابه
Synchrotron and neutron techniques in biological crystallography.
Synchrotron radiation (SR) techniques are continuously pushing the frontiers of wavelength range usage, smaller crystal sample size, larger protein molecular weight and complexity, as well as better diffraction resolution. The new research specialism of probing functional states directly in crystals, via time-resolved Laue and freeze trapping structural studies, has been developed, with a range...
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Neutron diffraction provides an experimental method of directly locating H atoms in proteins, a technique complementary to ultra-high-resolution X-ray diffraction. Three different types of neutron diffractometers for biological macromolecules have been constructed in Japan, France and the USA, and they have been used to determine the crystal structures of proteins up to resolution limits of 1.5...
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X-ray crystallography is the method of choice for determlning the 3-D structure of large macromolecules at a high enough resolution. The rate limiting step in structure determination is the crystallization itself. It takes anywhere between a few weeks to several years to obtain macromolecuiar crystals that yield good diffraction patterns. The theory of forces that promote and maintain crystal g...
متن کاملNeutron crystallography – Then and now *
Neutron crystallography began to be employed at the Bhabha Atomic Research Centre (BARC), Trombay, Mumbai in the early sixties. At that time, the technique, at BARC as well as elsewhere, was in a nascent state, with emphasis on building of instruments and development of crystallography software. Over the years, the Trombay group kept pace with the advancements in other parts of world and employ...
متن کاملCrystallography of Biological Macromolecules
Cyclohexene nucleic acids (CeNA) contain a cyclohexene ring instead of the normal -D-2’-deoxyribose. The cyclohexene oligonucleotide GTGTACAC was synthesized using phosphoramidite chemistry and standard protecting groups [1]. CeNA is stable against enzymatic degradation and induces RNaseH activity. CeNA also forms more stable duplexes with RNA than its natural analogues [2] [3]. Crystals of GTG...
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ژورنال
عنوان ژورنال: Nihon Kessho Gakkaishi
سال: 2004
ISSN: 0369-4585,1884-5576
DOI: 10.5940/jcrsj.46.193